ⓘ Agglutination-PCR

                                     

ⓘ Agglutination-PCR

Agglutination-PCR is an ultrasensitive solution-phase method for detecting antibodies. Antibodies bind to and agglutinate synthetic antigen–DNA conjugates, enabling ligation of the DNA strands and subsequent quantification by qPCR. Like other Immuno-PCR detection methods ADAP combines the specificity of antibody-antigen recognition and the sensitivity of PCR. ADAP detects zepto- to attomoles of antibodies in 2 μL of sample with a dynamic range spanning 5–6 orders of magnitude. For example, ADAP allows to detect anti-thyroglobulin autoantibodies from human patient plasma with a 1000-fold increased sensitivity over an FDA-approved radioimmunoassay. ADAP also allows to simultaneously detect multiple antibodies in one experiment, much more than ELISA or radioimmunoassay.

A study published in ACS Central science journal noted that this test method 10.000 times more effective than existing diagnostic methods.

Another advantage of the method ADAP is simplicity. It can be adapted to such cheap equipment, such as, for example, SlipChip. It does not require complex and expensive equipment, does not require the handling of hazardous radioactive reagents.

                                     
  • Agglutination is the clumping of particles. The word agglutination comes from the Latin agglutinare glueing to Agglutination is the process that occurs
  • Streptococcus pneumoniae, both as microscopic capsular swelling and macroscopic agglutination clumping visible with the naked eye It was initially an intellectual
  • using laboratory tests such as bacterial culture, PCR agar gel precipitation, ELISA and serum agglutination Antibiotic treatment can be attempted, but is
  • postmortem signs. Bacterial culture, immunofluorescence, PCR ELISA or slide agglutination tests SAT can be used to make a more definitive diagnosis
  • tissue samples or swabs and identified with PCR or immunofluorescence. ELISA and the Slide Agglutination Test are used for serological diagnosis. Antibiotics
  • Surround Optical Fiber Immunoassay SOFIA CD DVD based immunoassay Agglutination - PCR Optical immunoassay Yetisen A. K. 2013 Paper - based microfluidic
  • traditional method for determining the antigen formula is agglutination reactions on slides. The agglutination between the antigen and the antibody is made with
  • procedure for in field diagnosis is the detection of antibodies by latex agglutination LAT as it is quick and simple to run, and has a long shelf - life. Other
  • In 1897, A.E. Wright, a pathologist in British army, developed the agglutination test, diagnostic of the disease. In 1905, Zammit, a Maltese physician
  • capsulated strains show distinctive iridescence. The latex particle agglutination test LAT is a more sensitive method to detect H. influenzae than is
  • for its acronym in English, with agglutination techniques latex particles coated with antibody or antigen, agglutination techniques, articulation chromatography