ⓘ Epitope mapping

                                     

ⓘ Epitope mapping

Epitope mapping is the process of experimentally identifying the binding site, or "epitope", of an antibody on its target antigen. Identification and characterization of antibody binding sites aid in the discovery and development of new therapeutics, vaccines, and diagnostics. Epitope characterization can also help elucidate the mechanism of binding for an antibody and can strengthen intellectual property protection. Experimental epitope mapping data can be incorporated into robust algorithms to facilitate in silico prediction of B-cell epitopes based on sequence and/or structural data. Epitopes are generally divided into two classes: linear and conformational. Linear epitopes are formed by a continuous sequence of amino acids in a protein. Conformational epitopes are composed of amino acids that are discontinuous in the protein sequence but brought together upon three-dimensional protein folding. B-cell epitope mapping studies suggest that most interactions between antigens and antibodies, particularly autoantibodies and protective antibodies, rely on binding to conformational epitopes.

                                     
  • epitope mapping strategy developed to improve rapid mapping of conformational epitopes on structurally complex proteins. MHC class I and II epitopes can
  • related epitope and are binned together. Epitope binning is referenced in the literature under different names such as epitope mapping and epitope characterization
  • These are known as linear epitopes Antigen Linear epitope Epitope mapping finding on an antigen protein the epitope s for a specific antibody Goldsby
  • binding affinity less accurate. Antibody epitope mapping is used to find the specificity of an antibody. The epitope antibody binding site of antigens is
  • In 2006, Moutaftsi et al. demonstrated that epitope - mapping tools could accurately identify the epitopes responsible for 95 of the murine T - cell response
  • EpiMer and EpiMatrix epitope mapping tools. These were among the first motif - based and matrix - based, fully automated T cell epitope mapping tools. The team
  • an antigen is called an epitope There have been efforts in epitope mapping since many antibodies can bind the same epitope and levels of binding between
  • A peptide microarray also commonly known as peptide chip or peptide epitope microarray is a collection of peptides displayed on a solid surface, usually
  • included cytotoxicity of human lymphocytes and immunogen selection and epitope mapping for malaria vaccine development. Peter Perlmann died in 2005, but Eva
  • structure and chromosomal mapping of the mouse glucuronyltransferase - S involved in the biosynthesis of the HNK - 1 carbohydrate epitope Gene. 296 1 2 29 36
  • titin extends into the center of the sarcomeric M band. cDNA cloning, epitope mapping and immunoelectron microscopy of two titin - associated proteins Journal