ⓘ PIKFYVE

                                     

ⓘ PIKFYVE

The principal enzymatic activity of PIKfyve is to phosphorylate PtdIns3P to PtdIns3.5P2. PIKfyve activity is responsible for the production of both PtdIns3.5P2 and phosphatidylinositol 5-phosphate PtdIns5P. PIKfyve is a large protein, containing a number of functional domains and expressed in several spliced forms. The reported full-length mouse and human cDNA clones encode proteins of 2052 and 2098 amino acid residues, respectively. By directly binding membrane PtdIns3P, the FYVE finger domain of PIKfyve is essential in localizing the protein to the cytosolic leaflet of endosomes. Impaired PIKfyve enzymatic activity by dominant-interfering mutants, siRNA- mediated ablation or pharmacological inhibition causes lysosome enlargement and cytoplasmic vacuolation due to impaired PtdIns3.5P2 synthesis and impaired lysosome fission process and homeostasis. Thus, via PtdIns3.5P2 production, PIKfyve participates in several aspects of vesicular dynamics, thereby affecting a number of trafficking pathways that emanate from or traverse the endosomal system en route to the trans-Golgi network or later compartments along the endocytic pathway.

                                     
  • Protein VAC14 homolog, also known as Ar PIKfyve Associated Regulator of PIKfyve is a protein that in humans is encoded by the VAC14 gene. The content
  • PIKfyve called Ar PIKfyve VAC14, that scaffolds a ternary regulatory complex, known as the PAS complex from the first letters of PIKfyve Ar PIKfyve Sac3
  • heterologous overexpression of the enzymatically inactive PIKfyve point - mutant PIKfyve K1831E and PIKfyve silencing by small interfering RNAs. Such a role is
  • hetero - oligomer with Ar PIKfyve gene symbol, VAC14 and this binary complex associates with the phosphoinositide kinase PIKFYVE in a ternary PAS complex
  • dystrophy, is a rare form of corneal dystrophy. It is caused by mutations in PIKFYVE gene. Small opacities, some of which resemble flecks are scattered in
  • named after the four cysteine - rich proteins: Fab 1 yeast orthologue of PIKfyve YOTB, Vac 1 vesicle transport protein and EEA1, in which it has been
  • inositol ring, and can be converted to PtdIns 3, 5 P2 by the lipid kinase PIKfyve Both FYVE domains and PX domains found in proteins such as SNX1, Hrs
  • Jason Soans Aleric Finley Russell L Shisheva Assia Dec 2003 Active PIKfyve associates with and promotes the membrane attachment of the late endosome - to - trans - Golgi