ⓘ Tyrosylprotein sulfotransferase

                                     

ⓘ Tyrosylprotein sulfotransferase

Tyrosylprotein Sulfotransferase is the enzyme that catalyzes the sulfation reaction of protein tyrosines, a post-translational modification of proteins. It utilizes 3-Phosphoadenosine-5-phosphosulfate PAPS as the sulfonate donor and binds proteins with target tyrosine residues to eventually form the tyrosine O-sulfate ester group and the desulfonated 3’-phosphoadenosine-5’-phosphate PAP.

Tpst and the tyrosine sulfation is involved in a large number of biological and physiological processes. The sulfation of tyrosine has been found to be an important part of the inflammatory process, leukocyte traffic and cell count, the entrance of viral cells, and other cell-cell and protein-protein interactions. Choice for certain tyrosine residues requires the public residues of tyrosine and acidic residues within 5 or -5 residues of the target tyrosine. P-selectin ligand glycoprotein-1 PSGL-1 is widely studied as a substrate for tpst and the importance of sulfation in the PSGL-1 and its ability to bind its receptor. Another substrate for tpst, CC chemokine receptor 5 CCR5 co-receptor has attracted interest because of its role as a protein target for viral entry of HIV into cells. The importance of sulfation with CCR5 for HIV invasion have led to studies on tpst and CCR5, including characterization of the pattern of sulfation with CCR5. In addition to these two proteins, other known protein substrates include cholecystokinin CCK, factor V and factor VIII, gastrin, leeches enzyme hirudin, fibrinogen, complement component 4, follicle-stimulating hormone receptor FSHR and other chemokine and G-coupled protein receptors. A full, updated list can be found in UniProtKB.

                                     
  • sulfate 6 - O - sulfotransferase HS6ST1, HS6ST2, HS6ST3 N - deacetylase N - sulfotransferase NDST1, NDST2, NDST3, NDST4 tyrosylprotein sulfotransferase TPST1
  • in which a tyrosine residue of a protein is sulfated by a tyrosylprotein sulfotransferase TPST typically in the Golgi apparatus. Secreted proteins